Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine
Identifieur interne : 001926 ( Main/Exploration ); précédent : 001925; suivant : 001927Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine
Auteurs : P. Roccabianca [Italie] ; J. C. Woo [États-Unis] ; P. F. Moore [États-Unis]Source :
- Veterinary Immunology and Immunopathology [ 0165-2427 ] ; 2000.
English descriptors
- KwdEn :
- Teeft :
- Adult cats, Antigen expression, Beta, Canine, Cd3e, Cd8a, Cd8a expression, Cd8aa, Cd8aa chains, Cd8aa iels, Cd8ab, Cd8b, Cell antigens, Cell receptor, Cytometry, Cytospin preparations, Danilenko, Differential cell counts, Diffuse malt, Feline, Feline iels, Feline virus infection, Granular iels, Granular lymphocytes, Iels, Immunohistology, Immunol, Immunology, Immunopathology, Intestinal, Intestinal fragments, Intestine, Intraepithelial, Intraepithelial lymphocytes, Juvenile cats, Lamina, Lamina propria, Large intestine, Large percentage, Lefrancois, Leukocyte, Lpls, Lundqvist, Lymphocyte, Lymphocyte morphology, Lymphoid, Lymphoid tissue, Mabs, Malt, Minor subpopulation, Monoclonal antibodies, Mucosal, Murine, Other species, Peripheral blood, Phenotype, Phenotypic, Phenotypic analysis, Plasma cells, Propria, Regional specialization, Roccabianca, Similar percentages, Small intestine, Subpopulation, Tissue antigens, Tissue sections, Vassalli, Veterinary immunology.
Abstract
Abstract: Characterization of the feline intestinal mucosal associated lymphoid tissue (MALT) will facilitate investigation of intestinal disease in the cat and promote the cat as an animal model for a range of human diseases which involve the intestinal lymphoid tissue. This includes inflammatory bowel disease, viral and non-viral associated intestinal lymphomas and immunodeficiency associated syndromes. Morphologic and phenotypic characterization of the normal small intestinal diffuse MALT in 22 SPF cats was performed using flow cytometry and cytology on isolated intestinal leukocytes from the intra-epithelial and lamina proprial compartments, as well as immunohistology on tissues from the feline duodenum, jejunum and ileum. The intra-epithelial compartment (IEC) was dominated by lymphocytes (>85%) which frequently contained intracytoplasmic granules. The most striking findings in the IEC were the elevated percentages of CD8α+ lymphocytes (40%), presumed to express CD8αα chains, and CD4−/CD8− (double negative) lymphocytes (44%), and the consistent presence of a minor subpopulation of CD3+/CD11d+ IELs (6%). Small percentages of CD4+ lymphocytes (10%) were observed such that the IEL CD4:CD8 ratio (0.25) was low. The LPC also contained a majority of T cells and few plasma cells. However, this compartment had reduced percentages of CD8α+ lymphocytes (28%) and increased percentages of CD4+ lymphocytes (27%) relative to the IEC. However, the LPL CD4:CD8 ratio (1.0) remained low compared with the ratio in peripheral blood. In feline MALT, MHC class II expression was lower than in other peripheral lymphoid compartments. The results of this study provide important reference values for future investigations involving feline intestinal lymphocytes and demonstrates that the leukocyte distribution and phenotypic characteristics of the feline diffuse MALT appear largely similar to the murine, rat and human counterparts.
Url:
DOI: 10.1016/S0165-2427(00)00181-1
Affiliations:
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Roccabianca</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Istituto di Anatomia Patologica Veterinaria e Patologia Aviare, Facoltaı́ di Medicina Veterinaria, Via Celoria n. 10, 20133 Milan</wicri:regionArea><placeName><settlement type="city">Milan</settlement><region nuts="2">Lombardie</region></placeName></affiliation></author><author><name sortKey="Woo, J C" sort="Woo, J C" uniqKey="Woo J" first="J. C." last="Woo">J. C. Woo</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Pathology, Immunology and Microbiology, School of Veterinary Medicine, University of California at Davis, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Moore, P F" sort="Moore, P F" uniqKey="Moore P" first="P. F." last="Moore">P. F. 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This includes inflammatory bowel disease, viral and non-viral associated intestinal lymphomas and immunodeficiency associated syndromes. Morphologic and phenotypic characterization of the normal small intestinal diffuse MALT in 22 SPF cats was performed using flow cytometry and cytology on isolated intestinal leukocytes from the intra-epithelial and lamina proprial compartments, as well as immunohistology on tissues from the feline duodenum, jejunum and ileum. The intra-epithelial compartment (IEC) was dominated by lymphocytes (>85%) which frequently contained intracytoplasmic granules. The most striking findings in the IEC were the elevated percentages of CD8α+ lymphocytes (40%), presumed to express CD8αα chains, and CD4−/CD8− (double negative) lymphocytes (44%), and the consistent presence of a minor subpopulation of CD3+/CD11d+ IELs (6%). Small percentages of CD4+ lymphocytes (10%) were observed such that the IEL CD4:CD8 ratio (0.25) was low. The LPC also contained a majority of T cells and few plasma cells. However, this compartment had reduced percentages of CD8α+ lymphocytes (28%) and increased percentages of CD4+ lymphocytes (27%) relative to the IEC. However, the LPL CD4:CD8 ratio (1.0) remained low compared with the ratio in peripheral blood. In feline MALT, MHC class II expression was lower than in other peripheral lymphoid compartments. The results of this study provide important reference values for future investigations involving feline intestinal lymphocytes and demonstrates that the leukocyte distribution and phenotypic characteristics of the feline diffuse MALT appear largely similar to the murine, rat and human counterparts.</div></front></TEI><affiliations><list><country><li>Italie</li><li>États-Unis</li></country><region><li>Californie</li><li>Lombardie</li></region><settlement><li>Milan</li></settlement></list><tree><country name="Italie"><region name="Lombardie"><name sortKey="Roccabianca, P" sort="Roccabianca, P" uniqKey="Roccabianca P" first="P." last="Roccabianca">P. Roccabianca</name></region></country><country name="États-Unis"><region name="Californie"><name sortKey="Woo, J C" sort="Woo, J C" uniqKey="Woo J" first="J. C." last="Woo">J. C. Woo</name></region><name sortKey="Moore, P F" sort="Moore, P F" uniqKey="Moore P" first="P. F." last="Moore">P. F. Moore</name><name sortKey="Moore, P F" sort="Moore, P F" uniqKey="Moore P" first="P. F." last="Moore">P. F. Moore</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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